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Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status

Received: 6 April 2021     Published: 24 May 2021
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Abstract

Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (P<0.05), but they had nothing to do with iodine nutrition status. The C/G and C/C genotypes at the PTPN22 gene (1123 G>C) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.

Published in Science Journal of Public Health (Volume 9, Issue 3)
DOI 10.11648/j.sjph.20210903.13
Page(s) 83-91
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

PTPN22, Gene Polymorphism, Th17/Treg Cells and Factors, Iodine Nutritional Status, Graves' Disease

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    Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. (2021). Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Science Journal of Public Health, 9(3), 83-91. https://doi.org/10.11648/j.sjph.20210903.13

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    ACS Style

    Hou Zhenjiang; Mu Zhaoxin; Wang Cuicui; Feng Jing; Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(3), 83-91. doi: 10.11648/j.sjph.20210903.13

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    AMA Style

    Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(3):83-91. doi: 10.11648/j.sjph.20210903.13

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  • @article{10.11648/j.sjph.20210903.13,
      author = {Hou Zhenjiang and Mu Zhaoxin and Wang Cuicui and Feng Jing and Chen Yunxia and Ma Jinqun and Liu Chunyan},
      title = {Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status},
      journal = {Science Journal of Public Health},
      volume = {9},
      number = {3},
      pages = {83-91},
      doi = {10.11648/j.sjph.20210903.13},
      url = {https://doi.org/10.11648/j.sjph.20210903.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210903.13},
      abstract = {Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status
    AU  - Hou Zhenjiang
    AU  - Mu Zhaoxin
    AU  - Wang Cuicui
    AU  - Feng Jing
    AU  - Chen Yunxia
    AU  - Ma Jinqun
    AU  - Liu Chunyan
    Y1  - 2021/05/24
    PY  - 2021
    N1  - https://doi.org/10.11648/j.sjph.20210903.13
    DO  - 10.11648/j.sjph.20210903.13
    T2  - Science Journal of Public Health
    JF  - Science Journal of Public Health
    JO  - Science Journal of Public Health
    SP  - 83
    EP  - 91
    PB  - Science Publishing Group
    SN  - 2328-7950
    UR  - https://doi.org/10.11648/j.sjph.20210903.13
    AB  - Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou Medical College, Cangzhou, China

  • Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou Medical College, Cangzhou, China

  • Department of Medical Technology, Cangzhou Medical College, Cangzhou, China

  • Cangzhou Central Hospital, Cangzhou, China

  • Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China

  • Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China

  • Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China

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