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Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients

Received: 6 May 2020     Accepted: 22 May 2020     Published: 4 June 2020
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Abstract

Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.

Published in Science Journal of Public Health (Volume 8, Issue 3)
DOI 10.11648/j.sjph.20200803.13
Page(s) 77-82
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Foodborne Diseases, Broth Dilution, MIC, Salmonella, Results Analysis

References
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[4] Liu Guihua, Huang Xin, Zhang Weiyu, Gong Yunwei. Detection and analysis of drug resistance of Salmonella in stool of patients with food-borne diseases [J]. Chinese journal of public health engingeering 2018, v. 17 (01): 25-28.
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[15] Xu Yunmin, Du Yan, Dan Bin, etc.. Surveillance of drug resistance of Salmonella Spp. in CHINET from 2005 to 2014 [J]. Chinese Journal of Infection and chemotherapy, 2016, v. 16 (3): 294 -301.
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  • APA Style

    Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. (2020). Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Science Journal of Public Health, 8(3), 77-82. https://doi.org/10.11648/j.sjph.20200803.13

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    ACS Style

    Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci. J. Public Health 2020, 8(3), 77-82. doi: 10.11648/j.sjph.20200803.13

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    AMA Style

    Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci J Public Health. 2020;8(3):77-82. doi: 10.11648/j.sjph.20200803.13

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  • @article{10.11648/j.sjph.20200803.13,
      author = {Liu Guihua and Huang Xin and Zhang Weiyu and Wang Yanqiu},
      title = {Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients},
      journal = {Science Journal of Public Health},
      volume = {8},
      number = {3},
      pages = {77-82},
      doi = {10.11648/j.sjph.20200803.13},
      url = {https://doi.org/10.11648/j.sjph.20200803.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20200803.13},
      abstract = {Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients
    AU  - Liu Guihua
    AU  - Huang Xin
    AU  - Zhang Weiyu
    AU  - Wang Yanqiu
    Y1  - 2020/06/04
    PY  - 2020
    N1  - https://doi.org/10.11648/j.sjph.20200803.13
    DO  - 10.11648/j.sjph.20200803.13
    T2  - Science Journal of Public Health
    JF  - Science Journal of Public Health
    JO  - Science Journal of Public Health
    SP  - 77
    EP  - 82
    PB  - Science Publishing Group
    SN  - 2328-7950
    UR  - https://doi.org/10.11648/j.sjph.20200803.13
    AB  - Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

  • Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

  • Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

  • Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

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