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Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia

Received: 20 December 2014     Accepted: 4 January 2015     Published: 12 January 2015
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Abstract

Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard. Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.

Published in Science Journal of Public Health (Volume 3, Issue 1)
DOI 10.11648/j.sjph.20150301.16
Page(s) 31-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Malaria, Microscopy, RDT, P.falciparum, P.vivax

References
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[2] Federal Democratic Republic of Ethiopia Ministry of Health. Ethiopian National malaria indicator survey, 2007.
[3] Abeku T. Spatial and temporal variations of malaria epidemic risk in Ethiopia: factors involved and implications. J Acta Tropica. 2003; 87: 331-340.
[4] Martens P and Hall L. Malaria on the move: Human population movement and malaria transmission. Emerg Infect Dis. 2000; 6: 28-45
[5] Tulu AN. The Ecology of Malaria Health and Disease in Ethiopia. 1993; Pp.341-352.
[6] Moody A. A rapid dipstick antigen capture assay for the diagnosis of falciparum malaria. WHO Informal Consultation on Recent Advances in Diagnostic Techniques and Vaccines for Malaria. Bull World Health Organ.1996; 74:47-54.
[7] Neeru S, Man M, Mohan KS, Rajiv KM, Shweta S, et.al. Field and laboratory comparative evaluation of rapid malaria diagnostic tests versus traditiona and molecular techniques in India. J Malaria. 2010; 9:191.
[8] World Health Organization. Malaria (P. falciprum/P. vivax) W/B, RapiCard ™ Insta Test Cortez Diagnostic, Inc, 2009.
[9] Faisal RM. Laboratory Diagnosis of Malaria. 2009, Third Edition.
[10] Gillet P, David PD, Emmanuel B, Lieselotte C, Marjan VE, Jan J. Test characteristics of the SD FK80 Plasmodium falciparum/Plasmodium vivax malaria rapid diagnostic test in a non-endemic setting. J Malar 2009) 8: 26.
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[12] World Health Organization. A comparative study of three rapid diagnostic tests (RDTs) for malaria diagnosis in Oromia Regional State, Ethiopia. World Health Organization, 2000.
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[17] Biswas S, Tomar D and Rao DN. Investigation of the kinetics of histidine-rich protein 2 and of the antibody responses to this antigen, in a group of malaria patients from India. Ann Trop Med Parasitol. 2005; 99:553-562.
[18] Chansuda W, Mazie JB, Sinuon M, Awalludin S and Walther HW. A Review of Malaria Diagnostic Tools: Microscopy and Rapid Diagnostic Test (RDT). American J of Tropical Medicine and Hygiene. 2007; 77.
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  • APA Style

    Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. (2015). Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Science Journal of Public Health, 3(1), 31-36. https://doi.org/10.11648/j.sjph.20150301.16

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    ACS Style

    Sena Bayisa; Tadesse Kebede; Gessessew Bugssa; Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci. J. Public Health 2015, 3(1), 31-36. doi: 10.11648/j.sjph.20150301.16

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    AMA Style

    Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci J Public Health. 2015;3(1):31-36. doi: 10.11648/j.sjph.20150301.16

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  • @article{10.11648/j.sjph.20150301.16,
      author = {Sena Bayisa and Tadesse Kebede and Gessessew Bugssa and Megbaru Alemu},
      title = {Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia},
      journal = {Science Journal of Public Health},
      volume = {3},
      number = {1},
      pages = {31-36},
      doi = {10.11648/j.sjph.20150301.16},
      url = {https://doi.org/10.11648/j.sjph.20150301.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20150301.16},
      abstract = {Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia
    AU  - Sena Bayisa
    AU  - Tadesse Kebede
    AU  - Gessessew Bugssa
    AU  - Megbaru Alemu
    Y1  - 2015/01/12
    PY  - 2015
    N1  - https://doi.org/10.11648/j.sjph.20150301.16
    DO  - 10.11648/j.sjph.20150301.16
    T2  - Science Journal of Public Health
    JF  - Science Journal of Public Health
    JO  - Science Journal of Public Health
    SP  - 31
    EP  - 36
    PB  - Science Publishing Group
    SN  - 2328-7950
    UR  - https://doi.org/10.11648/j.sjph.20150301.16
    AB  - Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia

  • Department of Microbiology, Immunology and Parasitology (DMIP), School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia

  • Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia

  • Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia

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